Casper J T, Lauer S J, Kirchner P A, Gottschall J L, Camitta B M
Am J Clin Pathol. 1983 Nov;80(5):666-70. doi: 10.1093/ajcp/80.5.666.
Sixty cerebrospinal fluid (CSF) samples were obtained from 28 children with terminal deoxynucleotidyl transferase (TdT) positive acute lymphocytic leukemia (ALL). Cell morphology was evaluated using Wright's stained cytocentrifugation prepared slides. The presence of nuclear TdT was detected by an immunofluorescent (IF) assay. Evaluation of CSF mononuclear cells using these two methods simultaneously allowed us to differentiate between leukemic and nonleukemic pleocytosis. Agreement between cytomorphology and TdT in identifying CNS lymphoblasts was found in 55 of 60 samples. Seventy-two per cent of the TdT-positive samples were obtained from children with CSF cell counts less than 10 WBC/mm3. We recommend that these two methods be used in conjunction when evaluating CSF mononuclear cells from children with TdT positive ALL.
从28例末端脱氧核苷酸转移酶（TdT）阳性的急性淋巴细胞白血病（ALL）患儿中获取了60份脑脊液（CSF）样本。使用经瑞氏染色的细胞离心涂片评估细胞形态。通过免疫荧光（IF）测定法检测细胞核TdT的存在。同时使用这两种方法对脑脊液单核细胞进行评估，使我们能够区分白血病性和非白血病性细胞增多。在60份样本中的55份中发现细胞形态学与TdT在识别中枢神经系统成淋巴细胞方面具有一致性。72%的TdT阳性样本来自脑脊液细胞计数低于10个白细胞/mm³的儿童。我们建议，在评估TdT阳性ALL患儿的脑脊液单核细胞时，应联合使用这两种方法。
