Derevitskaya V A, Arbatsky N P, Kochetkov N K
Eur J Biochem. 1978 May 16;86(2):423-37. doi: 10.1111/j.1432-1033.1978.tb12325.x.
Twenty individual higher reduced oligosaccharides, having from seven to eleven monosaccharide units, were isolated after sodium borohydride degradation of blood-group substance H from pig stomach linings. Anion-exchange high-pressure liquid chromatography appears to be a very convenient and effective method for this kind of higher oligosaccharide mixtures separation. The oligosaccharide structures were determined by means of periodate oxidation, methylation analysis, partial acid and enzymic hydrolysis. It has been found that all the oligosaccharides investigated can be divided into four series. The oligosaccharides belonging to each series have the common oligosaccharide fragment to which terminal L-fucose and/or N-acetyl-D-glucosamine residues are attached. Comparison of all the oligosaccharide structures, including tri, penta and hexasaccharides described earlier, shows that the lower oligosaccharides represent the structural element of the higher oligosaccharides.
从猪胃黏膜血型物质H经硼氢化钠降解后分离出20种由7至11个单糖单元组成的高级还原寡糖。阴离子交换高压液相色谱法似乎是分离这类高级寡糖混合物非常方便且有效的方法。寡糖结构通过高碘酸盐氧化、甲基化分析、部分酸水解和酶水解来确定。已发现所有研究的寡糖可分为四个系列。属于每个系列的寡糖都有共同的寡糖片段，末端连接有L-岩藻糖和/或N-乙酰-D-葡萄糖胺残基。对所有寡糖结构（包括先前描述的三糖、五糖和六糖）的比较表明，低级寡糖是高级寡糖的结构单元。
