Wu A M
Mol Cell Biochem. 1984;61(2):131-41. doi: 10.1007/BF00222491.
The binding properties of Arachis hypogaea (PNA), Bauhinia purpurea alba (BPL), Maclura pomifera ( MPL ) and Sophora japonica (SJL) lectins were studied by quantitative precipitin and precipitin inhibition assays, demonstrating them to be most specific for DGal beta 1---- 3DGalNAc residues. Additionally, each lectin had its own binding characteristic such as different binding activities to DGal beta 1---- 4DGlcNAc or DGal beta 1---- 3DGlcNAc beta 1----linked oligosaccharides, and/or DGalNAc alpha 1----linked to the Ser or Thr of the protein moiety. These differential binding characteristics can be used for investigating fine differences of the carbohydrate structure of the glycoconjugates, especially those having DGal beta 1---- 3DGalNAc residues as terminal non-reducing ends.
通过定量沉淀和沉淀抑制试验研究了落花生（PNA）、白花羊蹄甲（BPL）、桑橙（MPL）和槐（SJL）凝集素的结合特性，结果表明它们对D - 半乳糖β1----3 - N - 乙酰半乳糖胺残基具有高度特异性。此外，每种凝集素都有其自身的结合特性，例如对D - 半乳糖β1----4 - N - 乙酰葡糖胺或D - 半乳糖β1----3 - N - 乙酰葡糖胺β1----连接的寡糖，和/或与蛋白质部分的丝氨酸或苏氨酸以α1----连接的N - 乙酰半乳糖胺具有不同的结合活性。这些不同的结合特性可用于研究糖缀合物碳水化合物结构的细微差异，尤其是那些以D - 半乳糖β1----3 - N - 乙酰半乳糖胺残基作为末端非还原端的糖缀合物。
