Biosynthesis of thromboxane B2 and 12-L-hydroxy-5,8,10-heptadecatrienoic acid in human platelets. Evidence for a common enzymatic pathway.

Human platelet microsomes convert prostaglandin H2 to thromboxane B2 and 12-L-hydroxy-5,8,10-heptadecatrienoic acid (12OH-17:3) in approximately equimolar amounts. The synthesizing activities of both products appear to go in parallel, both activities gradually decline upon storage and are equally destroyed by heat inactivation. Furthermore imidazole, a potent inhibitor of thromboxane synthetase activity, is an equally effective inhibitor of 12OH-17:3 formation in platelets. These results suggest that both thromboxane B2 and 120H-17:3 are derived from a common intermediate. We propose two alternative pathways for the conversion of prostaglandin H2 to thromboxane A2 and 12OH-17:3 in human platelets. The first pathway depicts thromboxane A2 as the common intermediate for the formation of both thromboxane B2 and 12OH-17:3. In the second pathway, prostaglanding H2 is converted to an activated intermediate which is converted to either thromboxane A2 or 12OH-17:3.