GTP水解对微管组装机制干扰的稳态理论。
Steady-state theory of the interference of GTP hydrolysis in the mechanism of microtubule assembly.
作者信息
Hill T L, Carlier M F
出版信息
Proc Natl Acad Sci U S A. 1983 Dec;80(23):7234-8. doi: 10.1073/pnas.80.23.7234.
Abstract
A model is presented for the interference of GTP hydrolysis in the mechanism of microtubule assembly. This model is suggested by previous results showing that both GTP and GDP are present at microtubule ends because of GTP hydrolysis and that tubulin does not bind to a GDP-bound end. The analytical theory developed here is aimed at calculation of the steady-state subunit flux at one end of the polymer. The GTP/GDP features just mentioned result in a nonlinear plot of the flux versus tubulin concentration. Microtubules are predicted to exhibit a different kinetic behavior below and above the critical concentration, which can be considered as a transition between two regimes.
摘要
本文提出了一个关于GTP水解对微管组装机制产生干扰的模型。该模型是基于先前的研究结果提出的，这些结果表明，由于GTP水解，GTP和GDP均存在于微管末端，并且微管蛋白不会与结合GDP的末端结合。本文所建立的分析理论旨在计算聚合物一端的稳态亚基通量。上述GTP/GDP特性导致通量与微管蛋白浓度的关系呈现非线性曲线。预计微管在临界浓度上下会表现出不同的动力学行为，这可以看作是两种状态之间的转变。
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