Nishikawa T, Strott C A
Steroids. 1983 Jan;41(1):105-19. doi: 10.1016/0039-128x(83)90021-1.
The following steroids were measured in their unconjugated and sulfoconjugated forms in plasma and in the outer and inner zones of the adrenal cortex of the guinea pig: pregnenolone, 17-hydroxypregnenolone, 21-hydroxypregnenolone, dehydroepiandrosterone and deoxycorticosterone. In plasma, pregnenolone and 21-hydroxypregnenolone were the predominant unconjugated steroids with concentrations 10-30 times higher than the other three steroids. Among the sulfoconjugated steroids, pregnenolone sulfate had a concentration 25-50 times higher than the other sulfoconjugates. For each steroid except 21-hydroxypregnenolone the sulfoconjugated form was present in a concentration 2-7 times higher than the unconjugated form. In the adrenal cortex, the content of 21-hydroxypregnenolone was significantly higher in the outer zone than in the inner zone and was present in amounts 3-100 times greater than the other unconjugated steroids in the outer zone. On the other hand, the content of pregnenolone was significantly greater in the inner zone than the outer zone, and was present in amounts 3-80 times greater than the other unconjugated steroids in the inner zone. With the exception of 21-hydroxypregnenolone and deoxycorticosterone, the steroid sulfoconjugates were significantly higher in the inner cortical zone. As in plasma, pregnenolone sulfate was the most abundant sulfoconjugated steroid. This report also describes preliminary studies concerning sulfurylated hydroxyl groups in different positions of 21-hydroxypregnenolone. The sulfoconjugate was prepared by using partially purified steroid sulfotransferase from the guinea pig adrenal. The results obtained indicated that of the total 21-hydroxypregnenolone conjugate formed, approximately 40% was the 21-sulfate and 20% the 3-sulfate, whereas 40% was non-hydrolyzable with the techniques used and was not further characterized.
在豚鼠的血浆以及肾上腺皮质的外层和内层区域中，对以下甾体化合物的游离形式和硫酸结合形式进行了测定：孕烯醇酮、17-羟孕烯醇酮、21-羟孕烯醇酮、脱氢表雄酮和脱氧皮质酮。在血浆中，孕烯醇酮和21-羟孕烯醇酮是主要的游离甾体化合物，其浓度比其他三种甾体化合物高10 - 30倍。在硫酸结合甾体化合物中，硫酸孕烯醇酮的浓度比其他硫酸结合物高25 - 50倍。除21-羟孕烯醇酮外，每种甾体化合物的硫酸结合形式的浓度比游离形式高2 - 7倍。在肾上腺皮质中，外层区域21-羟孕烯醇酮的含量显著高于内层区域，其含量比外层区域的其他游离甾体化合物高3 - 100倍。另一方面，孕烯醇酮在内层区域的含量显著高于外层区域，其含量比内层区域的其他游离甾体化合物高3 - 80倍。除21-羟孕烯醇酮和脱氧皮质酮外，皮质内层区域的甾体硫酸结合物含量显著更高。与血浆中一样，硫酸孕烯醇酮是含量最丰富的硫酸结合甾体化合物。本报告还描述了关于21-羟孕烯醇酮不同位置硫酸化羟基的初步研究。硫酸结合物是通过使用豚鼠肾上腺部分纯化的甾体磺基转移酶制备的。所得结果表明，在形成的总21-羟孕烯醇酮结合物中，约40%是21-硫酸盐，20%是3-硫酸盐，而40%用所使用的技术不可水解，未进一步表征。
