使用固定化酶的高效液相色谱法对个体血清胆汁酸进行高灵敏度测定。
A highly sensitive determination of individual serum bile acids using high-performance liquid chromatography with immobilized enzyme.
作者信息
Hasegawa S, Uenoyama R, Takeda F, Chuma J, Baba S, Kamiyama F, Iwakawa M, Fushimi M
出版信息
J Chromatogr. 1983 Nov 11;278(1):25-34. doi: 10.1016/s0378-4347(00)84752-0.
PMID:6582066
Abstract
We have developed a highly sensitive method for the simultaneous determination of individual bile acids in serum using high-performance liquid chromatography (HPLC) combined with immobilized 3 alpha-hydroxysteroid dehydrogenase. Both the HPLC column and the immobilized-enzyme column are suitable for use with alkaline solutions necessary in working with this enzyme system. With this method we were able to determine simultaneously fifteen different serum bile acids.
摘要
我们开发了一种高灵敏度方法，通过高效液相色谱（HPLC）结合固定化3α-羟基类固醇脱氢酶来同时测定血清中的单个胆汁酸。HPLC柱和固定化酶柱均适用于与该酶系统相关工作所需的碱性溶液。通过这种方法，我们能够同时测定十五种不同的血清胆汁酸。
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