采用柱后反应的高压液相色谱法对血清中游离型、甘氨酸结合型和牛磺酸结合型胆汁酸进行分组分离测定。
Assay of free and glycine- and taurine-conjugated bile acids in serum by high-pressure liquid chromatography by using post-column reaction after group separation.
作者信息
Onishi S, Itoh S, Ishida Y
出版信息
Biochem J. 1982 Apr 15;204(1):135-9. doi: 10.1042/bj2040135.
Abstract
An accurate and sensitive method that involves the group separations of serum bile acids (i.e. free and glycine- and taurine-conjugated bile acid fractions) by ion-exchange chromatography on piperidinohydroxypropyl-Sephadex LH-20 is described. Each group was then analysed by high-pressure liquid chromatography by using the post-column reaction technique with immobilized 3 alpha-hydroxy steroid dehydrogenase. The bile acid patterns in the umbilical venous serum samples were analysed by this method. Taurochenodeoxycholate predominated in the umbilical blood.
摘要
描述了一种准确且灵敏的方法，该方法涉及通过在哌啶羟丙基 - 葡聚糖凝胶LH - 20上进行离子交换色谱法对血清胆汁酸进行分组分离（即游离胆汁酸以及与甘氨酸和牛磺酸结合的胆汁酸组分）。然后，每组通过使用固定化3α - 羟基类固醇脱氢酶的柱后反应技术进行高压液相色谱分析。采用该方法分析了脐静脉血清样本中的胆汁酸模式。牛磺鹅去氧胆酸盐在脐血中占主导地位。
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本文引用的文献
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Serum bile acids in newborns and children.新生儿和儿童的血清胆汁酸
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