Weisinger G, Sachs L
EMBO J. 1983;2(12):2103-7. doi: 10.1002/j.1460-2075.1983.tb01709.x.
Macrophage and granulocyte-inducing (MGI) proteins regulate the growth and differentiation of myeloid hematopoietic cells. One class of these proteins (MGI-1) induces cell growth and another class (MGI-2) induces cell differentiation. Results obtained with DNA-cellulose column chromatography have shown that the differentiation-inducing protein MGI-2 can bind to double-stranded cellular DNA, but that there was no such binding under the same conditions by the growth-inducing protein MGI-1. DNA binding may thus be used to separate MGI-2 from MGI-1. The MGI-2 from mouse bound to DNA from mouse and calf. There were different elution peaks of the MGI-2 bound to DNA suggesting a heterogeneity of MGI-2 molecules, and the last peak eluted from the DNA cellulose column was enriched for one of the molecular forms of MGI-2. After one further step of purification by polyacrylamide gel electrophoresis, this molecular form of MGI-2 was active at a concentration of 6.5 X 10(-11) M. In normal development MGI-1 induces MGI-2. This induction of a DNA-binding differentiation-inducing protein by a growth-inducing protein is an efficient mechanism for the normal coupling of growth and differentiation. It is suggested that this may also be a mechanism for the normal coupling of growth and differentiation in other types of cells.
巨噬细胞和粒细胞诱导（MGI）蛋白调节髓系造血细胞的生长和分化。这类蛋白中的一类（MGI-1）诱导细胞生长，另一类（MGI-2）诱导细胞分化。通过DNA纤维素柱层析获得的结果表明，诱导分化的蛋白MGI-2能与双链细胞DNA结合，但在相同条件下，诱导生长的蛋白MGI-1则不能。因此，DNA结合可用于将MGI-2与MGI-1分离。来自小鼠的MGI-2能与来自小鼠和小牛的DNA结合。与DNA结合的MGI-2有不同的洗脱峰，表明MGI-2分子具有异质性，从DNA纤维素柱上洗脱的最后一个峰富含MGI-2的一种分子形式。经过聚丙烯酰胺凝胶电泳的进一步纯化步骤后，这种分子形式的MGI-2在浓度为6.5×10⁻¹¹ M时具有活性。在正常发育过程中，MGI-1诱导MGI-2。这种由诱导生长的蛋白诱导出一种结合DNA的诱导分化蛋白的现象，是生长与分化正常偶联的一种有效机制。有人提出，这也可能是其他类型细胞中生长与分化正常偶联的一种机制。
