培养的肾上皮细胞产生的一种蛋白质可抑制细胞生长和净钠离子通量。
Cell growth and net Na+ flux are inhibited by a protein produced by kidney epithelial cells in culture.
作者信息
Walsh-Reitz M M, Toback F G, Holley R W
出版信息
Proc Natl Acad Sci U S A. 1984 Feb;81(3):793-6. doi: 10.1073/pnas.81.3.793.
Proliferation of confluent kidney epithelial cell cultures (BSC-1 line) is inhibited by a protein (Mr approximately equal to 24,000) that is secreted by the cells. The mechanism of action of this growth inhibitor was sought by studying its effect on net Na+ flux because increased availability of Na+ in the culture medium had been shown to stimulate cell growth. The increase in cell Na+ content observed during stimulation of the growth after a medium change was attenuated in the presence of the purified inhibitor. Inhibition of both cell Na+ accumulation and growth in the presence of the protein was reversed completely by addition of NaCl to the medium. These results suggest that control of net Na+ flux and growth in kidney epithelial cells could be mediated, at least in part, by a secreted cellular protein.
汇合的肾上皮细胞培养物（BSC-1系）的增殖受到细胞分泌的一种蛋白质（分子量约为24,000）的抑制。通过研究其对净Na⁺通量的影响来探寻这种生长抑制剂的作用机制，因为已表明培养基中Na⁺可用性的增加会刺激细胞生长。在更换培养基刺激生长过程中观察到的细胞Na⁺含量增加，在存在纯化抑制剂的情况下会减弱。向培养基中添加NaCl可完全逆转蛋白质存在时对细胞Na⁺积累和生长的抑制。这些结果表明，肾上皮细胞中净Na⁺通量和生长的控制至少部分可由一种分泌的细胞蛋白质介导。
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