Walsh-Reitz M M, Toback F G, Holley R W
Proc Natl Acad Sci U S A. 1984 Feb;81(3):793-6. doi: 10.1073/pnas.81.3.793.
Proliferation of confluent kidney epithelial cell cultures (BSC-1 line) is inhibited by a protein (Mr approximately equal to 24,000) that is secreted by the cells. The mechanism of action of this growth inhibitor was sought by studying its effect on net Na+ flux because increased availability of Na+ in the culture medium had been shown to stimulate cell growth. The increase in cell Na+ content observed during stimulation of the growth after a medium change was attenuated in the presence of the purified inhibitor. Inhibition of both cell Na+ accumulation and growth in the presence of the protein was reversed completely by addition of NaCl to the medium. These results suggest that control of net Na+ flux and growth in kidney epithelial cells could be mediated, at least in part, by a secreted cellular protein.
汇合的肾上皮细胞培养物(BSC-1系)的增殖受到细胞分泌的一种蛋白质(分子量约为24,000)的抑制。通过研究其对净Na⁺通量的影响来探寻这种生长抑制剂的作用机制,因为已表明培养基中Na⁺可用性的增加会刺激细胞生长。在更换培养基刺激生长过程中观察到的细胞Na⁺含量增加,在存在纯化抑制剂的情况下会减弱。向培养基中添加NaCl可完全逆转蛋白质存在时对细胞Na⁺积累和生长的抑制。这些结果表明,肾上皮细胞中净Na⁺通量和生长的控制至少部分可由一种分泌的细胞蛋白质介导。