Leung M K, Stefano G B
Proc Natl Acad Sci U S A. 1984 Feb;81(3):955-8. doi: 10.1073/pnas.81.3.955.
An acid extract of pedal ganglia of the mollusc Mytilus edulis was fractionated by high-pressure liquid chromatography with a reverse-phase column. Peak fractions with retention times of those of [Met]- and [Leu]enkephalin were subjected to binding assays in both invertebrate and vertebrate tissues. The results showed that these fractions have the same binding activities as authentic enkephalins. Peptides from these fractions were purified by high-pressure liquid chromatography under isocratic conditions. Sequential amino acid analyses showed that these peptides have the same primary structures as [Met]- and [Leu]enkephalin. These results with M. edulis suggest that invertebrates possess an enkephalinergic system similar to that of higher organisms.
用反相柱通过高压液相色谱法对贻贝（Mytilus edulis）足神经节的酸提取物进行分级分离。保留时间与[甲硫氨酸]-和[亮氨酸]脑啡肽相同的峰级分在无脊椎动物和脊椎动物组织中进行结合测定。结果表明，这些级分具有与天然脑啡肽相同的结合活性。通过等度条件下的高压液相色谱法对这些级分中的肽进行纯化。顺序氨基酸分析表明，这些肽具有与[甲硫氨酸]-和[亮氨酸]脑啡肽相同的一级结构。贻贝的这些结果表明，无脊椎动物拥有与高等生物类似的脑啡肽能系统。
