从软体动物神经节中纯化阿片肽。
Purification of opioid peptides from molluscan ganglia.
作者信息
Stefano G B, Leung M
出版信息
Cell Mol Neurobiol. 1982 Dec;2(4):347-52. doi: 10.1007/BF00710854.
Abstract
- Acid extracts from pedal ganglia of Mytilus edulis was fractionated by high-pressure liquid chromatography (HPLC). 2. Components were recovered which had the same retention times as met- and leu-enkephalin. 3. When tested in an in vitro assay, these components inhibited dopamine-stimulated adenylate cyclase activity in a naloxone-reversible manner. 4. These results suggest that molluscan neural tissue contains opioid peptides similar, perhaps identical, to the mammalian enkephalins.
摘要
- 对紫贻贝足神经节的酸性提取物进行高压液相色谱（HPLC）分离。2. 回收了与甲硫氨酸脑啡肽和亮氨酸脑啡肽保留时间相同的成分。3. 在体外试验中进行测试时，这些成分以纳洛酮可逆的方式抑制多巴胺刺激的腺苷酸环化酶活性。4. 这些结果表明，软体动物神经组织含有与哺乳动物脑啡肽相似、可能相同的阿片肽。
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Purification of opioid peptides from molluscan ganglia.从软体动物神经节中纯化阿片肽。
Cell Mol Neurobiol. 1982 Dec;2(4):347-52. doi: 10.1007/BF00710854.
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Isolation of molluscan opioid peptides.软体动物阿片样肽的分离
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引用本文的文献
1
Isolation and identification of enkephalins in pedal ganglia of Mytilus edulis (Mollusca).紫贻贝（软体动物）足神经节中脑啡肽的分离与鉴定。
Proc Natl Acad Sci U S A. 1984 Feb;81(3):955-8. doi: 10.1073/pnas.81.3.955.
2
Alterations in high-affinity binding characteristics and levels of opioids in invertebrate ganglia during aging: evidence for an opioid compensatory mechanism.衰老过程中无脊椎动物神经节中阿片类药物的高亲和力结合特性和水平的变化：阿片类药物补偿机制的证据。
Cell Mol Neurobiol. 1984 Jun;4(2):143-55. doi: 10.1007/BF00711001.
本文引用的文献
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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
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Enkephalin-like immunoreactivity in the pedal ganglion of Mytilus edulis (Bivalvia) and its proximity to dopamine-containing structures.紫贻贝（双壳纲）足神经节中的脑啡肽样免疫反应性及其与含多巴胺结构的接近程度。
Cell Tissue Res. 1983;230(1):147-53. doi: 10.1007/BF00216035.
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4
Characterization of the dopamine stimulated adenylate cyclase in the pedal ganglia of Mytilus edulis: interactions with etorphine, beta-endorphin, DALA, and methionine enkephalin.紫贻贝足神经节中多巴胺刺激的腺苷酸环化酶的特性：与埃托啡、β-内啡肽、DALA和甲硫氨酸脑啡肽的相互作用
Cell Mol Neurobiol. 1981 Mar;1(1):57-68. doi: 10.1007/BF00736039.
5
Opioid inhibition of dopamine release from nervous tissue of Mytilus edulis and Octopus bimaculatus.阿片类物质对紫贻贝和双斑蛸神经组织中多巴胺释放的抑制作用。
Science. 1981 Aug 21;213(4510):928-30. doi: 10.1126/science.6266017.
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