Nicolas J C, Boussioux A M, Boularan A M, Descomps B, Crastes de Paulet A
Anal Biochem. 1983 Nov;135(1):141-5. doi: 10.1016/0003-2697(83)90742-x.
A rapid and very sensitive enzymatic assay of estrogens was developed using the transhydrogenase reaction of the human estradiol-17 beta dehydrogenase and bioluminescence for the quantification of NADH accumulated during the first reaction. The assay requires two steps: first, the addition of transhydrogenase buffer, and a few minutes later the addition of the bioluminescence reagent. The method can determine 0.1 to 50 pg of estrogens in the assay, in 1 h, with a coefficient of variation of 5%.
利用人雌二醇-17β脱氢酶的转氢酶反应和生物发光技术，开发了一种快速且非常灵敏的雌激素酶法测定方法，用于定量第一次反应中积累的NADH。该测定需要两个步骤：首先，加入转氢酶缓冲液，几分钟后加入生物发光试剂。该方法在1小时内可测定0.1至50 pg的雌激素，变异系数为5%。
