Mounib N, Sultan C, Bringer J, Hedon B, Nicolas J C, Cristol P, Bressot N, Descomps B
INSERM Unité 58, Hôpital Lapeyronie, Montpellier, France.
J Steroid Biochem. 1988 Nov;31(5):861-5. doi: 10.1016/0022-4731(88)90297-x.
Estrone and estradiol (E1 + E2) concentrations in saliva were compared with four other parameters of estrogen status in five normal ovulatory women and ten FSH stimulated women selected for an in vitro fertilization program. E1 + E2 in saliva, plasma, and urine were assessed by a rapid, specific and sensitive enzymatic assay using bioluminescence. The free fraction of plasma estradiol was determined by equilibrium dialysis and total plasma estradiol by conventional radioimmunoassay. The pattern of E1 + E2 variation in saliva was similar to that of free plasma estradiol and the two parameters were correlated in both spontaneous and stimulated cycles. However, the lower correlation coefficient (r = 0.52, P less than 0.001) in spontaneous cycles compared with the high (r = 0.96, P less than 0.001) in the stimulated cycles shows that salivary E1 + E2 could be representative of plasma free estradiol in stimulated cycles but not in normal cycles. The free fraction of plasma estradiol reproduced the variation of total plasma estradiol in spontaneous as well as in FSH stimulated cycles and both parameters were strongly correlated (r = 0.91, P less than 0.001 and r = 0.90, P less than 0.001), respectively. The data show that salivary E1 + E2 concentrations are highly representative of the free fraction of E2 in plasma and at a lesser extend (r = 0.72, P less than 0.001) of total plasma E2 in FSH stimulated cycles.
在五名正常排卵女性和十名因体外受精计划而接受促卵泡生成素（FSH）刺激的女性中，比较了唾液中雌酮和雌二醇（E1 + E2）的浓度与雌激素状态的其他四个参数。采用生物发光法，通过一种快速、特异且灵敏的酶促测定法评估唾液、血浆和尿液中的E1 + E2。通过平衡透析法测定血浆雌二醇的游离部分，通过传统放射免疫测定法测定血浆总雌二醇。唾液中E1 + E2的变化模式与血浆游离雌二醇相似，并且在自然周期和刺激周期中这两个参数均具有相关性。然而，与刺激周期中较高的相关系数（r = 0.96，P < 0.001）相比，自然周期中的相关系数较低（r = 0.52，P < 0.001），这表明唾液中的E1 + E2在刺激周期中可代表血浆游离雌二醇，但在正常周期中则不然。血浆雌二醇的游离部分在自然周期以及FSH刺激周期中均再现了血浆总雌二醇的变化，并且这两个参数分别具有强相关性（r = 0.91，P < 0.001和r = 0.90，P < 0.001）。数据表明，唾液中E1 + E2的浓度高度代表血浆中E2的游离部分，在FSH刺激周期中对血浆总E2的代表性稍低（r = 0.72，P < 0.001）。
