Toda N, Nakajima M, Nishimura K, Miyazaki M
Cardiovasc Res. 1984 Mar;18(3):174-82. doi: 10.1093/cvr/18.3.174.
Helically-cut strips of dog cerebral, coronary, mesenteric, renal and femoral arteries contracted with prostaglandin (PG) F2 alpha or K+ responded to amrinone (10(-5) to 10(-4) mol X litre-1) with relaxation, which was not influenced by treatment with propranolol, atropine, cimetidine, aminophylline or aspirin. The contractile response of mesenteric arteries to transmural electrical stimulation (2, 5 and 20 Hz) and noradrenaline was attenuated by amrinone (3 X 10(-5) and 10(-4) mol X litre-1); the attenuation of the response to nerve stimulation and noradrenaline did not significantly differ. Ca2+-induced contractions in mesenteric arteries exposed to Ca2+-free media and depolarised by excess K+ were inhibited by amrinone, and the inhibition could not be reversed by the addition of excess Ca2+. Treatment with amrinone potentiated the relaxant responses of mesenteric arteries to adenosine but did not alter the response to isoprenaline. Amrinone in concentrations sufficient to produce moderate and marked relaxation did not significantly alter the content of cyclic AMP in mesenteric arteries. Attenuation by amrinone of the contractile response to transmural stimulation, noradrenaline and Ca2+ and the relaxation of a variety of arteries induced by amrinone may not be due to interference with the transmembrane influx of Ca2+ and intracellular accumulation of cyclic AMP but to a nonspecific action on arterial smooth muscle. Amrinone appears to increase the metabolic vasodilatation by potentiating the vasodilator action of adenosine.
犬脑动脉、冠状动脉、肠系膜动脉、肾动脉和股动脉的螺旋形切片，用前列腺素（PG）F2α或K⁺收缩后，对氨力农（10⁻⁵至10⁻⁴mol·L⁻¹）产生舒张反应，该反应不受普萘洛尔、阿托品、西咪替丁、氨茶碱或阿司匹林处理的影响。氨力农（3×10⁻⁵和10⁻⁴mol·L⁻¹）可减弱肠系膜动脉对跨壁电刺激（2、5和20Hz）和去甲肾上腺素的收缩反应；对神经刺激和去甲肾上腺素反应的减弱无显著差异。在无钙培养基中暴露且因过量K⁺而 depolarised 的肠系膜动脉中，Ca²⁺诱导的收缩被氨力农抑制，且加入过量Ca²⁺不能逆转该抑制作用。氨力农处理可增强肠系膜动脉对腺苷的舒张反应，但不改变对异丙肾上腺素的反应。足以产生中度和明显舒张的氨力农浓度不会显著改变肠系膜动脉中环磷酸腺苷（cAMP）的含量。氨力农对跨壁刺激、去甲肾上腺素和Ca²⁺收缩反应的减弱以及氨力农诱导的多种动脉舒张，可能不是由于干扰Ca²⁺的跨膜内流和cAMP的细胞内积累，而是对动脉平滑肌的非特异性作用。氨力农似乎通过增强腺苷的血管舒张作用来增加代谢性血管舒张。 （注：原文中“depolarised”可能有误，推测应为“去极化”相关含义，这里按原文翻译）
