Pollack V A, Fidler I J
J Natl Cancer Inst. 1982 Jul;69(1):137-41.
The purpose of these studies was to investigate whether young (3-wk-old) nude mice, which lack functional T-lymphocytes and demonstrate low natural killer cell activity, could serve as in vivo models for the selection of metastatic subpopulations of cells from heterogeneous allogeneic melanomas. Three-week-old BALB/cAnN or N:NIH(S) nude mice received iv injections of single cells harvested from either the B16 or K-1735 melanoma. Individual pulmonary metastases were harvested 4 weeks later and implanted sc into nude mice to expand the population. Cells from each of these metastases colonized in the lungs of 3-week-old nude mice and 6-week-old normal syngeneic mice with significantly higher efficiency than did cells from the parent tumors. It was concluded that, in addition to being a useful in vivo model for ascertaining the metastatic potential of neoplasms, the healthy young nude mouse could be used for selecting and maintaining tumor cell variants of high metastatic potential room heterogeneous allogeneic tumors.
这些研究的目的是调查缺乏功能性T淋巴细胞且自然杀伤细胞活性较低的3周龄裸鼠是否可作为体内模型，用于从异基因同种黑色素瘤中选择具有转移能力的细胞亚群。3周龄的BALB/cAnN或N:NIH(S)裸鼠经静脉注射从B16或K - 1735黑色素瘤中获取的单个细胞。4周后采集单个肺转移灶，并皮下植入裸鼠以扩大细胞群体。这些转移灶中的细胞在3周龄裸鼠和6周龄同基因正常小鼠肺中的定植效率明显高于亲本肿瘤细胞。得出的结论是，健康的幼龄裸鼠除了可作为确定肿瘤转移潜能的有用体内模型外，还可用于从异基因同种肿瘤中选择和维持具有高转移潜能的肿瘤细胞变体。
