Inoue T, Shimono M, Yamamura T, Saito I, Watanabe O, Kawahara H
Oral Surg Oral Med Oral Pathol. 1984 Apr;57(4):398-407. doi: 10.1016/0030-4220(84)90159-2.
Two cases of acinic cell carcinoma arising in the glossopalatine glands were examined with the electron microscope by means of conventional thin sectioning and freeze-fracturing. Light and electron microscopic observation revealed that the tumors consisted of three types of cells: serous-type, ductlike, and vacuolated cells. Serous-type cells had numerous secretory granules, some of which were discharged into the intercellular spaces. Ductlike cells were smaller, usually lacked secretory granules, and were similar to intercalated duct cells. Vacuolated cells had electron-opaque vacuoles in the cytoplasm. Our findings supported the hypothesis that acinic cell carcinoma may represent a neoplasm of multipotential duct cells which have differentiated mainly into granulated serous cells. Freeze-fracture images of this tumor revealed that tight junctions were composed of ten or more interlinked strands with elongation of basal frontier strands. These findings suggested that the junctional morphology of the tumor resembled that of developing salivary glands and was associated with the degree of cellular differentiation.
通过传统薄切片和冷冻断裂技术，利用电子显微镜对两例发生于腭舌腺的腺泡细胞癌进行了检查。光镜和电镜观察显示，肿瘤由三种类型的细胞组成：浆液型、导管样和空泡状细胞。浆液型细胞有大量分泌颗粒，其中一些排入细胞间隙。导管样细胞较小，通常缺乏分泌颗粒，与闰管细胞相似。空泡状细胞的细胞质中有电子致密的空泡。我们的研究结果支持以下假说：腺泡细胞癌可能代表一种多能导管细胞的肿瘤，这些细胞主要分化为颗粒状浆液细胞。该肿瘤的冷冻断裂图像显示，紧密连接由十条或更多相互连接的链组成，基底前沿链伸长。这些发现表明，肿瘤的连接形态与发育中的唾液腺相似，且与细胞分化程度有关。
