Poduslo J F, Berg C T, Dyck P J
Proc Natl Acad Sci U S A. 1984 Mar;81(6):1864-6. doi: 10.1073/pnas.81.6.1864.
Quiescent Schwann cells in the distal segment of the permanently transected nerve produced basal levels of the major myelin glycoprotein, P0, in the absence of myelin assembly. Low levels of P0 could be detected at 35 days after transection by autoradiographic analysis of radioiodinated lectin binding after protein separation by high-resolution sodium dodecyl sulfate pore gradient electrophoresis and by fluorographic analysis after electrophoresis of [3H]fucose- and [3H]mannose-labeled glycoproteins after incorporation into endoneurial slices. Immunoreactivity to P0 in the transected nerve could also be demonstrated with antisera against P0 as evaluated by direct "immune overlay" after electrophoresis. These results indicate that the requirement for continuing signals from appropriate axons to make detectable amounts of myelin-specific proteins and glycolipids is not absolute. Schwann cells, therefore, like oligodendrocytes, can synthesize myelin components in the absence of neuronal influence, although information from neuronal elements probably is required for myelin assembly by Schwann cells and for myelin compaction by oligodendrocytes.
在永久性横断神经的远侧段，静止的雪旺细胞在没有髓鞘组装的情况下产生主要髓鞘糖蛋白P0的基础水平。在横断后35天，通过高分辨率十二烷基硫酸钠孔径梯度电泳分离蛋白质后对放射性碘化凝集素结合进行放射自显影分析，以及在将[3H]岩藻糖和[3H]甘露糖标记的糖蛋白掺入神经内膜切片后进行电泳荧光分析，可检测到低水平的P0。通过电泳后直接“免疫覆盖”评估，用抗P0抗血清也可证明横断神经中对P0的免疫反应性。这些结果表明，来自适当轴突的持续信号对于产生可检测量的髓鞘特异性蛋白质和糖脂并非绝对必要。因此，雪旺细胞与少突胶质细胞一样，在没有神经元影响的情况下也能合成髓鞘成分，尽管雪旺细胞进行髓鞘组装以及少突胶质细胞进行髓鞘压实可能需要来自神经元成分的信息。
