用于真核生物扩增基因原位筛选的菌落杂交法。
Colony hybridization method for screening in situ of eukaryotic amplified genes.
作者信息
Malpièce Y, Michel M L, Carloni G
出版信息
Experientia. 1984 May 15;40(5):483-5. doi: 10.1007/BF01952397.
PMID:6586493
Abstract
A new method for autoradiographic screening of amplified genes in cellular clones is described. The main feature of the device is to keep viable cells from each clone, which can subsequently be regrown. The availability of this biochemical screening method allows screening for recombinants harboring unselectable markers as well.
摘要
本文描述了一种用于细胞克隆中扩增基因放射自显影筛选的新方法。该装置的主要特点是保留每个克隆的活细胞，这些细胞随后可以再次生长。这种生化筛选方法的可用性也使得能够筛选携带不可选择标记的重组体。
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