Pourcel C, Louise A, Gervais M, Chenciner N, Dubois M F, Tiollais P
J Virol. 1982 Apr;42(1):100-5. doi: 10.1128/JVI.42.1.100-105.1982.
Mouse L cells transformed with recombinant plasmids carrying hepatitis B virus (HBV) DNA fragments were used to study the transcription of the viral surface antigen gene (gene S). An HBV-specific, polyadenylated, 2.3-kilobase RNA was mapped on the HBV genome. This RNA hybridized with approximately 75% of the genome and excluded the region of the HBV core antigen gene (gene C). The 2.3-kilobase RNA species was present only in cell lines that produced hepatitis B surface antigen. An HBV-specific 2.3-kilobase RNA was also detected in human hepatoma cell line PLC/PRF/5 which produced hepatitis B surface antigen. A study of gene S expression in the transformed mouse L cells allowed us to localize the regions of initiation and termination of gene S transcription. Our results strongly suggest that the 2.3-kilobase RNA molecule is the mRNA of the major polypeptide of the envelope, which carries the viral surface antigen determinants.
用携带乙肝病毒（HBV）DNA片段的重组质粒转化的小鼠L细胞，用于研究病毒表面抗原基因（S基因）的转录。一种HBV特异性的、多聚腺苷酸化的2.3千碱基RNA被定位在HBV基因组上。这种RNA与大约75%的基因组杂交，并排除了HBV核心抗原基因（C基因）区域。2.3千碱基的RNA种类仅存在于产生乙肝表面抗原的细胞系中。在产生乙肝表面抗原的人肝癌细胞系PLC/PRF/5中也检测到一种HBV特异性的2.3千碱基RNA。对转化的小鼠L细胞中S基因表达的研究使我们能够定位S基因转录的起始和终止区域。我们的结果强烈表明，2.3千碱基的RNA分子是包膜主要多肽的mRNA，它携带病毒表面抗原决定簇。
