El-Fiky S M, Taha Y M
Gegenbaurs Morphol Jahrb. 1978;124(1):127-32.
The effect of one massive dose of cytoxan (150 mg/kg), as an alkylating agent, on the morphology of sarcoma 180 cells was followed after 1, 3, 6, 12, 24, 48, 96, 120, and 168 h. After 1 h the cells of treated tumour showed clumping and a decrease in their size. In addition, statistical analysis manifested significant decrease in all phases of mitotic figures. All mitotic figures were completely stopped after 3 h from cytoxan treatment. After 6 and 12 h from cytoxan treatment the hyaline degenerations became more marked in the treated tumour tissue. Chromosomal bridge formation, nuclei aberrations and polynuclear cells were more prominent in 24, 48, and 96 h from cytoxan treatment. Increase in the nuclear size and hyaline degeneration were observed in treated section of 120 and 168 h.
作为一种烷化剂，单次大剂量注射环磷酰胺（150毫克/千克）后，在1、3、6、12、24、48、96、120和168小时观察其对肉瘤180细胞形态的影响。注射后1小时，经处理的肿瘤细胞出现聚集且体积减小。此外，统计分析表明有丝分裂相的各个阶段均显著减少。环磷酰胺处理3小时后，所有有丝分裂相完全停止。环磷酰胺处理6小时和12小时后，处理后的肿瘤组织中透明变性变得更加明显。环磷酰胺处理24、48和96小时后，染色体桥形成、细胞核畸变和多核细胞更为突出。在处理120小时和168小时的切片中观察到细胞核大小增加和透明变性。
