来自猕猴肝脏的硫氰酸酶。II. 酶动力学及作用机制方面
Rhodanese from Cercopithecus aethiops (vervet monkey) liver. II. Aspects of enzyme kinetics and mechanism of action.
作者信息
Janse van Rensburg L, Schabort J C
出版信息
Int J Biochem. 1984;16(5):547-51. doi: 10.1016/0020-711x(84)90173-3.
PMID:6586595
Abstract
Initial velocity kinetic studies were undertaken and certain kinetic parameters ( KmSSO2 -3 = 3.1 X 10(-3) M and Vmax = 153.85 U/ml/min.) were determined and the mechanism identified as a ping-pong (double displacement) mechanism. Competitive inhibition of rhodanese by both substrates, viz. thiosulphate and cyanide, provides additional evidence of Ping-Pong Bi-Bi mechanism for this transferase.
摘要
进行了初始速度动力学研究，确定了某些动力学参数（KmSSO2 -3 = 3.1×10⁻³ M，Vmax = 153.85 U/ml/min），并确定该机制为乒乓（双置换）机制。两种底物，即硫代硫酸盐和氰化物对硫氰酸酶的竞争性抑制，为该转移酶的乒乓双底物机制提供了额外证据。
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