小鼠白血病细胞中含硒转运RNA的存在情况。
Occurrence of selenium-containing tRNAs in mouse leukemia cells.
作者信息
Ching W M
出版信息
Proc Natl Acad Sci U S A. 1984 May;81(10):3010-3. doi: 10.1073/pnas.81.10.3010.
Abstract
Selenium incorporation into the polynucleotide structures of tRNAs has been documented in several microorganisms. In the present study, selenium-containing species were isolated from bulk tRNA preparations from 75Se-labeled mouse leukemia cells. The major 75Se-labeled species was similar in size and exhibited the same sensitivity to ribonuclease as did Escherichia coli tRNAs. The chromatographic properties of the intact major selenium-containing tRNA species indicated it to be very hydrophobic in character. The selenium component that is unstable at neutral-to-alkaline pH but is relatively stable at acid pH is not an esterified selenoamino acid. HPLC analysis of enzymic digests of the major selenium-containing species detected selenium-containing hydrophobic products (probably selenonucleosides ). These properties strongly suggest that the selenium in the mouse leukemia-cell tRNAs is present in the form of a selenium-modified nucleoside.
摘要
在几种微生物中，已证明硒可掺入转运核糖核酸（tRNA）的多核苷酸结构中。在本研究中，从用75Se标记的小鼠白血病细胞的大量tRNA制剂中分离出含硒物种。主要的75Se标记物种大小相似，并且对核糖核酸酶的敏感性与大肠杆菌tRNA相同。完整的主要含硒tRNA物种的色谱特性表明其具有很强的疏水性。在中性至碱性pH下不稳定但在酸性pH下相对稳定的硒成分不是酯化的硒代氨基酸。对主要含硒物种的酶解产物进行的高效液相色谱（HPLC）分析检测到含硒的疏水产物（可能是硒代核苷）。这些特性强烈表明，小鼠白血病细胞tRNA中的硒以硒修饰核苷的形式存在。
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