Hole A
Acta Anaesthesiol Scand. 1984 Jun;28(3):280-6. doi: 10.1111/j.1399-6576.1984.tb02061.x.
The in vitro effects of six anaesthesia-related drugs and five stress-related serum factors on monocyte-mediated cytolysis and thymidine uptake in mitogen-(PHA)-stimulated lymphocytes have been studied. Thiopentone depressed both the monocyte and lymphocyte function in a dose-dependent way. However, at thiopentone concentrations which may be present in the serum after a single intravenous anaesthesia induction dose, the monocyte depression was moderate and depression of the lymphocytes was not observed. The other drugs tested, fentanyl, morphine, pancuronium, diazepam and bupivacaine, did not alter the cellular functions significantly. Prostaglandin-E2 in concentrations of 10(-6) and 10(-7) M markedly depressed monocyte-mediated cytolysis. Cortisol, catecholamines and serotonin did not alter this function. However, a synergistic depressive effect of the combination of prostaglandin-E2 and cortisol was observed. The proliferative response of PHA-stimulated lymphocytes was depressed by cortisol in concentrations of 2200 nmol/l and 1100 nmol/1 Again, there was a marked synergistic effect of the combination of cortisol and prostaglandin-E2, while prostaglandin-E2 alone, catecholamines and serotonin did not influence the PHA-response. A possible explanation for the depression of monocyte-mediated cytolysis and lymphocyte-thymidine uptake during and after surgery under general anaesthesia may be the combined effect of endocrine and local stress factors.
研究了六种麻醉相关药物和五种应激相关血清因子对单核细胞介导的细胞溶解以及对丝裂原（PHA）刺激的淋巴细胞摄取胸苷的体外作用。硫喷妥钠以剂量依赖的方式抑制单核细胞和淋巴细胞功能。然而，在单次静脉麻醉诱导剂量后血清中可能存在的硫喷妥钠浓度下，单核细胞的抑制作用中等，未观察到淋巴细胞的抑制作用。所测试的其他药物，芬太尼、吗啡、泮库溴铵、地西泮和布比卡因，对细胞功能没有显著改变。浓度为10(-6)和10(-7) M的前列腺素E2显著抑制单核细胞介导的细胞溶解。皮质醇、儿茶酚胺和血清素未改变此功能。然而，观察到前列腺素E2和皮质醇联合具有协同抑制作用。浓度为2200 nmol/l和1100 nmol/1的皮质醇抑制PHA刺激的淋巴细胞的增殖反应。同样，皮质醇和前列腺素E2联合具有显著的协同作用，而单独的前列腺素E2、儿茶酚胺和血清素不影响PHA反应。全身麻醉手术期间及术后单核细胞介导的细胞溶解和淋巴细胞胸苷摄取受到抑制的一个可能解释可能是内分泌和局部应激因素的联合作用。
