Saitou E
Bull Tokyo Med Dent Univ. 1984 Mar;31(1):33-50.
The effects of fluoroacetate on the ameloblasts were studied in the rat incisor. Fluoroacetate is an inhibitor of tricarboxylic acid cycle and accumulation of citrate occurred in the animal tissues due to fluoroacetate administration. In the present study, fluoroacetate injection caused severe morphologic changes in the ameloblasts. The most prominent change was observed in the mitochondria. Reduction of the mitochondrial matrix density was the earliest change followed by varying degrees of matrix swelling. Loss of the matrix granules and disintegration of the cristae were also observed. The difference in the mitochondrial activities in regard to the citrate metabolism was found between the matrix formation stage and the maturation stage in the ameloblasts. Extensive dilatation of the rough-surfaced endoplasmic reticulum and grossly enlarged vacuoles were found mainly in the early maturation stage at 12 and 24 hours after the fluoroacetate administration. These abnormally large vacuoles seemed to be caused by the water stored within the endoplasmic reticulum cisternae. Accumulation of plasma citrate and decrease of ionized calcium concentration in the whole blood were observed in the fluoroacetate treated group. These findings suggest that fluoroacetate may cause the lowering of the function of the ameloblasts through the suppression of cell energy production and that both the secretion of the matrix and the calcification of the enamel may be inhibited.
在大鼠切牙中研究了氟乙酸对成釉细胞的影响。氟乙酸是三羧酸循环的抑制剂，由于给予氟乙酸，动物组织中出现柠檬酸盐积累。在本研究中，注射氟乙酸导致成釉细胞出现严重的形态学变化。最显著的变化出现在线粒体中。线粒体基质密度降低是最早出现的变化，随后是不同程度的基质肿胀。还观察到基质颗粒的丢失和嵴的解体。在成釉细胞的基质形成阶段和成熟阶段，发现线粒体在柠檬酸盐代谢方面的活性存在差异。主要在给予氟乙酸后12小时和24小时的早期成熟阶段发现粗面内质网广泛扩张和液泡明显增大。这些异常大的液泡似乎是由内质网池内储存的水引起的。在氟乙酸处理组中观察到血浆柠檬酸盐积累和全血中离子钙浓度降低。这些发现表明，氟乙酸可能通过抑制细胞能量产生导致成釉细胞功能降低，并且可能抑制基质分泌和釉质钙化。
