Simon W E, Albrecht M, Trams G, Dietel M, Hölzel F
J Natl Cancer Inst. 1984 Aug;73(2):313-21. doi: 10.1093/jnci/73.2.313.
Individually different growth responses of 10 cell lines newly derived from metastasizing mammary carcinomas were determined by cell counts in experimental incubations with the steroid hormones 17 beta-estradiol, progesterone, testosterone, hydrocortisone (cortisol), the antiestrogenic compound tamoxifen, or prolactin. Of 7 cell lines derived from ductal carcinomas, 5 were stimulated by prolactin. The growth of 4 of 7 cell lines established from the tumors of postmenopausal or ovariectomized patients was enhanced by doses of testosterone, which are in the range of the physiologic serum level. The proliferation of 5 cell lines was promoted by hydrocortisone in the physiologic concentration of 100 nM, supporting the notion that concentrations of testosterone or hydrocortisone normally present in body fluids may facilitate the in vivo growth of breast cancer. The in vitro growth of cells derived from tumors after relapse under treatment with medroxyprogesterone acetate or tamoxifen was markedly enhanced by progesterone or tamoxifen (CAS: 10540-29-1) in concentrations corresponding to therapeutical serum levels and in accordance with in vivo resistance to the endocrine therapy applied before cell sampling. The results of this suggest the occurrence of positive endocrine selection mechanisms operating in vivo on human mammary tumor cell populations.
通过对10种新建立的源自转移性乳腺癌的细胞系，在含有甾体激素17β - 雌二醇、孕酮、睾酮、氢化可的松（皮质醇）、抗雌激素化合物他莫昔芬或催乳素的实验培养中进行细胞计数，确定了它们各自不同的生长反应。在源自导管癌的7种细胞系中，有5种受到催乳素的刺激。从绝经后或去卵巢患者肿瘤中建立的7种细胞系中的4种，其生长受到睾酮剂量的增强，这些睾酮剂量处于生理血清水平范围内。5种细胞系的增殖受到生理浓度为100 nM的氢化可的松的促进，这支持了体液中正常存在的睾酮或氢化可的松浓度可能促进乳腺癌体内生长的观点。在醋酸甲羟孕酮或他莫昔芬治疗后复发的肿瘤所衍生细胞的体外生长，在与治疗血清水平相对应的浓度下，被孕酮或他莫昔芬（CAS：10540 - 29 - 1）显著增强，并且与细胞采样前应用的内分泌治疗的体内抗性一致。这一结果表明在人类乳腺肿瘤细胞群体中存在体内起作用的阳性内分泌选择机制。
