De Martelaere D, Brugmans M, Cassiman J J, Van den Berghe H
Oncology. 1984;41(4):264-9. doi: 10.1159/000225835.
A human osteosarcoma derived cell line was treated with phorbol-12-myristate-13-acetate and was subsequently cloned extensively. 27 clones were examined for their initial rate of aggregation, their attachment to homo- and heterotypic performed cell layers, and the size of their 24-hour aggregates. Quantitative differences for these three adhesive parameters were observed between the clones. Based on these findings five clones were studied in more detail. The adhesive differences between these clones were stable, reproducible, and dependent on the assay system. This human clonal system will be a valuable tool to study the molecular nature of the adhesive properties of these cells.
一种源自人骨肉瘤的细胞系用佛波醇-12-肉豆蔻酸酯-13-乙酸酯处理，随后进行广泛克隆。检测了27个克隆的初始聚集速率、它们与同型和异型预形成细胞层的附着情况以及它们24小时聚集体的大小。在这些克隆之间观察到这三个黏附参数的定量差异。基于这些发现，对五个克隆进行了更详细的研究。这些克隆之间的黏附差异是稳定的、可重复的，并且依赖于检测系统。这个人类克隆系统将成为研究这些细胞黏附特性分子本质的有价值工具。
