杜兴氏肌营养不良症患者的成纤维细胞与基质分离的速率增加。
Increased rate of cell-substratum detachment of fibroblasts from patients with Duchenne muscular dystrophy.
作者信息
Kent C
出版信息
Proc Natl Acad Sci U S A. 1983 May;80(10):3086-90. doi: 10.1073/pnas.80.10.3086.
When skin fibroblasts from patients with Duchenne muscular dystrophy were treated with trypsin in the presence of divalent cations, they detached more rapidly from the substratum than did fibroblasts from normal individuals of similar age, sex, and passage number. This difference was observed when either the time of incubation or trypsin concentration was varied. The ease of detachment of both normal and dystrophic fibroblasts varied somewhat with culture age and plating density, although detachment was always greater for fibroblasts from dystrophic individuals. If the trypsin treatment was carried out in the absence of divalent cations, both types of fibroblasts detached rapidly from the substratum, suggesting that a divalent-cation dependent cell-substratum adhesion mechanism is altered in Duchenne muscular dystrophy fibroblasts.
当在二价阳离子存在的情况下用胰蛋白酶处理杜兴氏肌营养不良症患者的皮肤成纤维细胞时，它们从基质上脱离的速度比年龄、性别和传代次数相似的正常个体的成纤维细胞更快。当孵育时间或胰蛋白酶浓度变化时，均观察到这种差异。正常和营养不良的成纤维细胞的脱离难易程度会随培养时间和接种密度有所不同，尽管营养不良个体的成纤维细胞的脱离总是更明显。如果在没有二价阳离子的情况下进行胰蛋白酶处理，两种类型的成纤维细胞都会迅速从基质上脱离，这表明在杜兴氏肌营养不良症成纤维细胞中，一种依赖二价阳离子的细胞 - 基质粘附机制发生了改变。
Zotero 插件