Boon M E, Veldhuizen R W, Ruinaard C, Snieders M W, Kwee W S
Acta Cytol. 1984 Jul-Aug;28(4):443-9.
The qualitative features of the intracytoplasmic vacuoles occurring in the cells of pleural malignant mesothelioma and of metastatic carcinoma exfoliated in pleural fluid were studied. There were important differences in the two types of vacuoles. In mesotheliomas, the vacuoles were centrally situated, small and regular in size; these vacuoles stained positively with fat stains and always negatively with the periodic acid-Schiff stain. In routine cytology, these lipid-containing vacuoles can best be seen in air-dried May-Grünwald-Giemsa-stained smears and are almost invisible in wet-fixed Papanicolaou-stained smears. In metastatic carcinoma, the vacuoles were irregular in size, noncentral in location and often positive with mucin stains. Electron microscopy was used in one case to further elucidate the staining patterns of the vacuoles. This study proves that the analysis of the qualitative features of vacuoles of malignant cells exfoliated in pleural fluid can be of great value in the distinction between metastatic carcinoma and mesothelioma.
对胸膜恶性间皮瘤细胞及胸膜液中脱落的转移性癌细胞内出现的胞质空泡的定性特征进行了研究。这两种类型的空泡存在重要差异。在间皮瘤中，空泡位于细胞中央，体积小且大小规则；这些空泡用脂肪染色呈阳性，而高碘酸希夫染色始终呈阴性。在常规细胞学检查中，这些含脂质的空泡在空气干燥的May-Grünwald-Giemsa染色涂片中最易观察到，而在湿固定的巴氏染色涂片中几乎不可见。在转移性癌中，空泡大小不规则，位置不在中央，且黏液染色常呈阳性。在1例中使用电子显微镜进一步阐明空泡的染色模式。本研究证明，分析胸膜液中脱落的恶性细胞空泡的定性特征对区分转移性癌和间皮瘤具有重要价值。
