Fazio M, Airoldi M, Negri L, Marchesa P, Gandolfo S
J Maxillofac Surg. 1984 Aug;12(4):153-6. doi: 10.1016/s0301-0503(84)80236-2.
Following earlier personal studies indicating that cryosurgery stimulates several non-specific immunological parameters, mainly those of the cell-mediated component, in patients with squamous-cell carcinoma of the oral cavity, inhibition of leukocyte migration (evaluated by means of the LIF test) was investigated, using a heterologous squamous-cell tumour extract, before and 7 and 14 days after a single cryotherapy session in 14 patients (11 in stages I and II, 3 in stages III and IV). Increased inhibition was observed after 7 days in 8 subjects (57.2%)-72.7% in stage I-II patients. In two cases, reactivity appeared for the first time. In 7/8 cases, this increase persisted, though at lower levels, until the 14th day. The conclusion is drawn that cryosurgery can stimulate specific delayed hypersensitivity in stage I-II squamous-cell carcinoma of mouth.
早期的个人研究表明，冷冻手术可刺激口腔鳞状细胞癌患者的多种非特异性免疫参数，主要是细胞介导成分的参数。在此基础上，我们对14例患者（11例I期和II期，3例III期和IV期）进行了研究，在单次冷冻治疗前以及治疗后7天和14天，使用异种鳞状细胞瘤提取物，通过白细胞移动抑制试验（LIF试验）评估白细胞迁移抑制情况。8名受试者（57.2%）在7天后出现抑制增加，I-II期患者中这一比例为72.7%。有2例患者首次出现反应性。在7/8的病例中，这种增加一直持续到第14天，不过水平有所降低。由此得出结论，冷冻手术可刺激I-II期口腔鳞状细胞癌患者的特异性迟发型超敏反应。
