用高效液相色谱法测定血清中的亚胺培南和西司他丁。
Determination of imipenem and cilastatin in serum by high-pressure liquid chromatography.
作者信息
Myers C M, Blumer J L
出版信息
Antimicrob Agents Chemother. 1984 Jul;26(1):78-81. doi: 10.1128/AAC.26.1.78.
Abstract
Imipenem and cilastatin concentrations in serum were determined by using reverse-phase high-pressure liquid chromatography. Serum samples were stabilized with 0.5 M morpholineethanesulfonic acid buffer (pH 6.0) and subjected to ultrafiltration before chromatography. The elution solvent consisted of water or potassium phosphate buffered to pH 2.5 and methanol. The imipenem and cilastatin peaks were detected at 300 and 220 nm, respectively. Recovery from serum was 99% for both imipenem and cilastatin, and the limits of detectability for the two compounds were 0.3 and 0.5 microgram/ml, respectively. The assay may be readily applied to pharmacokinetic analysis of imipenem and cilastatin biodisposition in patients.
摘要
采用反相高效液相色谱法测定血清中亚胺培南和西司他丁的浓度。血清样本用0.5M吗啉乙磺酸缓冲液（pH 6.0）稳定，并在色谱分析前进行超滤。洗脱溶剂由pH 2.5的水或磷酸钾缓冲液和甲醇组成。亚胺培南和西司他丁的峰分别在300nm和220nm处检测到。亚胺培南和西司他丁从血清中的回收率均为99%，两种化合物的检测限分别为0.3和0.5微克/毫升。该测定法可很容易地应用于患者中亚胺培南和西司他丁生物处置的药代动力学分析。
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