Cathcart R, Schwiers E, Saul R L, Ames B N
Proc Natl Acad Sci U S A. 1984 Sep;81(18):5633-7. doi: 10.1073/pnas.81.18.5633.
Thymine glycol is a DNA damage product of ionizing radiation and other oxidative mutagens. In an attempt to find a noninvasive assay for oxidative DNA damage in individuals, we have developed an HPLC assay for free thymine glycol and thymidine glycol in urine. Our results indicate that humans excrete about 32 nmol of the two glycols per day. Rats, which have a higher specific metabolic rate and a shorter life span, excrete about 15 times more thymine glycol plus thymidine glycol per kg of body weight than do humans. We present evidence that thymine glycol and thymidine glycol are likely to be derived from repair of oxidized DNA, rather than from alternative sources such as the diet or bacterial flora. This noninvasive assay of DNA oxidation products may allow the direct testing of current theories which relate oxidative metabolism to the processes of aging and cancer in man.
胸腺嘧啶乙二醇是电离辐射及其他氧化性诱变剂导致的DNA损伤产物。为了找到一种针对个体氧化DNA损伤的非侵入性检测方法，我们开发了一种用于检测尿液中游离胸腺嘧啶乙二醇和胸苷乙二醇的高效液相色谱法。我们的结果表明，人类每天排泄约32纳摩尔的这两种乙二醇。大鼠具有较高的比代谢率和较短的寿命，每千克体重排泄的胸腺嘧啶乙二醇加胸苷乙二醇比人类多约15倍。我们提供的证据表明，胸腺嘧啶乙二醇和胸苷乙二醇可能源自氧化DNA的修复，而非来自饮食或细菌菌群等其他来源。这种对DNA氧化产物的非侵入性检测方法可能允许直接检验当前将氧化代谢与人类衰老和癌症过程相关联的理论。
