Ovtscharoff W
Histochemistry. 1978 May 16;55(4):341-5. doi: 10.1007/BF00508801.
A method for demonstration of electron-dense particles within clear synaptic vesicles from various areas of the CNS as well as from neuromuscular junctions of rat is described. Electron-dense granules of 70-250 A were visible in the center of the synaptic vesicles, or in some cases excentrically situated and bound to the vesicular membrane. Digestion with proteolytic enzymes lead to a negative reaction, whereas treatment with hyaluronidase and neuraminidase, as well as the lipid extraction had no effect. Based on the obtained data, it may be assumed that this method manifests the proteinaceous structures.
本文描述了一种用于显示来自大鼠中枢神经系统不同区域以及神经肌肉接头的清亮突触小泡内电子致密颗粒的方法。在突触小泡中心可见70 - 250埃的电子致密颗粒，在某些情况下，这些颗粒偏心定位并与泡膜结合。用蛋白水解酶消化会导致阴性反应，而用透明质酸酶和神经氨酸酶处理以及脂质提取则没有效果。根据所获得的数据，可以推测这种方法显示的是蛋白质结构。
