Zoller L C
Anat Rec. 1984 Oct;210(2):279-91. doi: 10.1002/ar.1092100203.
The structure and enzymatic content of rat and hamster preovulatory follicles were examined using morphologic, qualitative, and quantitative cytochemical techniques. Interfollicular structure in both types was similar but lipid droplet distribution differed. The rat theca contained many more droplets than the hamster, while the hamster membrana granulosa contained more droplets than the rat. delta 5-3 beta-hydroxysteroid dehydrogenase (3 beta OHD) activity and glucose-6-phosphate dehydrogenase (G-6-PD) Types IH and IIH generation were measured using quantitative cytochemistry. Rat theca and granulosa contained more 3 beta OHD activity than found in comparable regions in hamster. G-6-PD Type IH and Type IIH generation were the same in comparable regions of rat and hamster, but in rat there was more Type IIH than IH, while the converse existed in hamster. These differences and their relationship to follicular steroid production are discussed.
运用形态学、定性及定量细胞化学技术，对大鼠和仓鼠排卵前卵泡的结构及酶含量进行了检测。两种类型卵泡的卵泡间结构相似，但脂滴分布有所不同。大鼠卵泡膜含有的脂滴比仓鼠多得多，而仓鼠颗粒膜含有的脂滴比大鼠多。采用定量细胞化学方法测定了δ5-3β-羟基类固醇脱氢酶（3βOHD）活性以及葡萄糖-6-磷酸脱氢酶（G-6-PD）的ⅠH型和ⅡH型生成情况。大鼠的卵泡膜和颗粒层所含3βOHD活性比仓鼠相应区域的活性更高。大鼠和仓鼠相应区域的G-6-PDⅠH型和ⅡH型生成情况相同，但大鼠中ⅡH型比ⅠH型更多，而仓鼠情况则相反。讨论了这些差异及其与卵泡类固醇生成的关系。
