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小鼠β-珠蛋白主要基因5'-侧翼和结构基因序列的α-鹅膏蕈碱不敏感转录与mRNA表达相关。

alpha-Amanitin-insensitive transcription of mouse beta major-globin 5'-flanking and structural gene sequences correlates with mRNA expression.

作者信息

Carlson D P, Ross J

出版信息

Proc Natl Acad Sci U S A. 1984 Dec;81(24):7782-6. doi: 10.1073/pnas.81.24.7782.

DOI:10.1073/pnas.81.24.7782
PMID:6595660
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC392236/
Abstract

A small proportion of the RNAs of mouse reticulocytes consists of beta major-globin mRNA sequences linked to sequences transcribed from the 5'-flanking region of the beta major-globin gene. These upstream RNAs are polyadenylylated and contain 700-800 nucleotides, and their 5' regions are heterogeneous. RNAs with similar or identical 5' regions are transcribed in cell-free extracts from a circular mouse beta major-globin gene template. Synthesis of most of the upstream RNAs in vitro is not inhibited by low levels (1 microgram/ml) of alpha-amanitin, indicating that they are transcribed by an enzyme(s) different from RNA polymerase II. During culture of mouse erythroleukemia cells with dimethyl sulfoxide, globin mRNA and upstream RNAs accumulate with similar kinetics. In contrast, upstream RNAs are not detected in hemin-treated cells.

摘要

小鼠网织红细胞的一小部分RNA由与从β-珠蛋白基因5'侧翼区域转录的序列相连的β-珠蛋白mRNA序列组成。这些上游RNA是聚腺苷酸化的，含有700 - 800个核苷酸，其5'区域是异质的。具有相似或相同5'区域的RNA在来自环状小鼠β-珠蛋白基因模板的无细胞提取物中被转录。体外大多数上游RNA的合成不受低水平（1微克/毫升）α-鹅膏蕈碱的抑制，这表明它们是由不同于RNA聚合酶II的一种或多种酶转录的。在用二甲基亚砜培养小鼠红白血病细胞的过程中，珠蛋白mRNA和上游RNA以相似的动力学积累。相比之下，在经血红素处理的细胞中未检测到上游RNA。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47a1/392236/a9b5db3a6960/pnas00625-0124-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47a1/392236/cb064e655104/pnas00625-0122-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47a1/392236/7ae5fc76307a/pnas00625-0123-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47a1/392236/abd3a83e0256/pnas00625-0123-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47a1/392236/9bd934b1f870/pnas00625-0124-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47a1/392236/a9b5db3a6960/pnas00625-0124-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47a1/392236/cb064e655104/pnas00625-0122-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47a1/392236/7ae5fc76307a/pnas00625-0123-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47a1/392236/abd3a83e0256/pnas00625-0123-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47a1/392236/9bd934b1f870/pnas00625-0124-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47a1/392236/a9b5db3a6960/pnas00625-0124-b.jpg

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本文引用的文献

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J Mol Biol. 1982 Dec 5;162(2):345-64. doi: 10.1016/0022-2836(82)90531-9.
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DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract.腺病毒基因在可溶性全细胞提取物中的DNA依赖性转录。
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Isolation and partial characterization of the Drosophila alcohol dehydrogenase gene.果蝇乙醇脱氢酶基因的分离与部分特性分析
与β珠蛋白主基因启动子相互作用的核蛋白在诱导后12小时内开始在MEL细胞中积累，并且该启动子的RNA拷贝在体外能成功竞争它们的结合。
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Minor transcription initiation events indicate that both human mitochondrial promoters function bidirectionally.微小转录起始事件表明，人类线粒体的两个启动子都具有双向功能。
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Structural and transcriptional analysis of DNA sequences flanking genes that encode 19 kilodalton zeins.编码19千道尔顿醇溶蛋白的基因侧翼DNA序列的结构与转录分析
Mol Gen Genet. 1987 Apr;207(1):90-8. doi: 10.1007/BF00331495.
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