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源自K562细胞和正常人胚胎中mRNA帽位点上游的ε珠蛋白基因转录本。

Epsilon globin gene transcripts originating upstream of the mRNA cap site in K562 cells and normal human embryos.

作者信息

Alan M, Grindlay G J, Stefani L, Paul J

出版信息

Nucleic Acids Res. 1982 Sep 11;10(17):5133-47. doi: 10.1093/nar/10.17.5133.

DOI:10.1093/nar/10.17.5133
PMID:6292831
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC320860/
Abstract

RNA transcribed from the human epsilon globin gene was studied in K562 cells and human embryos of 5-10 weeks gestation. Using both primer extension and S1 analysis RNA molecules were found which extend 53 +/- 1 bp, 55 +/- 1 bp and 270 +/- 1 bp upstream from the first coding base and are colinear with the gene. The first pair of molecules represent transcripts initiating at the canonical cap site but the other species represents a transcript over 200 nucleotides longer which initiates upstream of the CAAT and ATAA boxes.

摘要

在K562细胞和妊娠5 - 10周的人类胚胎中研究了从人类ε珠蛋白基因转录而来的RNA。使用引物延伸和S1分析，发现RNA分子从第一个编码碱基向上游延伸53±1 bp、55±1 bp和270±1 bp，并且与该基因共线。第一对分子代表在典型帽位点起始的转录本，但其他种类代表一种长超过200个核苷酸的转录本，其在CAAT盒和ATAA盒的上游起始。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4f6/320860/5cc5a7a3ef09/nar00386-0049-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4f6/320860/a3542ee4aa62/nar00386-0042-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4f6/320860/c08a508c59c9/nar00386-0044-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4f6/320860/48e9db375345/nar00386-0045-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4f6/320860/e0a1a66411fa/nar00386-0046-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4f6/320860/c91fccdfc5e2/nar00386-0048-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4f6/320860/5cc5a7a3ef09/nar00386-0049-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4f6/320860/a3542ee4aa62/nar00386-0042-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4f6/320860/c08a508c59c9/nar00386-0044-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4f6/320860/48e9db375345/nar00386-0045-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4f6/320860/e0a1a66411fa/nar00386-0046-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4f6/320860/c91fccdfc5e2/nar00386-0048-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4f6/320860/5cc5a7a3ef09/nar00386-0049-a.jpg

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1
Epsilon globin gene transcripts originating upstream of the mRNA cap site in K562 cells and normal human embryos.源自K562细胞和正常人胚胎中mRNA帽位点上游的ε珠蛋白基因转录本。
Nucleic Acids Res. 1982 Sep 11;10(17):5133-47. doi: 10.1093/nar/10.17.5133.
2
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引用本文的文献

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3
Alternative sites of transcription initiation upstream of the canonical cap site in human gamma-globin and beta-globin genes.

本文引用的文献

1
A transcription map for the rabbit beta-globin gene.兔β-珠蛋白基因的转录图谱。
Cell. 1981 Feb;23(2):573-84. doi: 10.1016/0092-8674(81)90153-7.
2
The size of the transcriptional units of the avian globin genes defined at the pre-messenger RNA level.在信使核糖核酸前体水平上确定的鸟类珠蛋白基因转录单位的大小。
J Mol Biol. 1980 Jul 15;140(4):481-504. doi: 10.1016/0022-2836(80)90267-3.
3
Multiple mRNAs are generated from the chicken lysozyme gene.鸡溶菌酶基因可产生多种信使核糖核酸。
人类γ-珠蛋白基因和β-珠蛋白基因中位于典型帽位点上游的转录起始替代位点。
Nucleic Acids Res. 1984 Feb 24;12(4):1811-20. doi: 10.1093/nar/12.4.1811.
4
alpha-Amanitin-insensitive transcription of mouse beta major-globin 5'-flanking and structural gene sequences correlates with mRNA expression.小鼠β-珠蛋白主要基因5'-侧翼和结构基因序列的α-鹅膏蕈碱不敏感转录与mRNA表达相关。
Proc Natl Acad Sci U S A. 1984 Dec;81(24):7782-6. doi: 10.1073/pnas.81.24.7782.
5
Inducible transcription of five globin genes in K562 human leukemia cells.K562人白血病细胞中五个珠蛋白基因的可诱导转录。
Proc Natl Acad Sci U S A. 1983 Sep;80(18):5515-9. doi: 10.1073/pnas.80.18.5515.
6
Nuclear beta-globin RNA transcripts initiated in vitro with gamma-thio nucleoside triphosphate.用γ-硫代核苷三磷酸在体外起始的核β-珠蛋白RNA转录本。
Cell Biophys. 1983 Dec;5(4):255-64. doi: 10.1007/BF02788624.
7
The chromatin structure of the human epsilon globin gene: nuclease hypersensitive sites correlate with multiple initiation sites of transcription.人类ε珠蛋白基因的染色质结构：核酸酶超敏位点与多个转录起始位点相关。
Nucleic Acids Res. 1984 Dec 11;12(23):9191-204. doi: 10.1093/nar/12.23.9191.
8
The primary structure of the larval beta 1-globin gene of Xenopus laevis and its flanking regions.非洲爪蟾幼虫β1-珠蛋白基因的一级结构及其侧翼区域。
Nucleic Acids Res. 1984 Oct 25;12(20):7705-19. doi: 10.1093/nar/12.20.7705.
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EMBO J. 1984 Jun;3(6):1263-72. doi: 10.1002/j.1460-2075.1984.tb01961.x.
10
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6
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Annu Rev Biochem. 1981;50:349-83. doi: 10.1146/annurev.bi.50.070181.002025.
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