Hedenborg G, Norman A, Samuelson K
Scand J Clin Lab Invest. 1984 Dec;44(8):765-7. doi: 10.3109/00365518409083642.
A recently developed enzymatic method of determining unsulphated 7 alpha-hydroxylated bile acids in serum was evaluated for the analysis of bile acids in urine after hydrolysis of the sulphate esters. Results obtained using the enzymatic method were compared to those obtained by radioimmunoassay of 7 alpha-hydroxylated bile acids in urine samples from patients. Results of the enzymatic method were also compared to results of gas-liquid chromatography of individual bile acids after group separation of the bile acids on Sephadex-DEAP-LH-20. There was significant correlation between the enzymatic method and radioimmunoassay for bile acid determination in urine. The analysis of different conjugates after group separation of bile acids revealed no unspecific reactions when compared to gas-liquid chromatography. The reference range for 7 alpha-hydroxylated urinary bile acids was in agreement with earlier published results.
对最近开发的一种测定血清中未硫酸化的7α-羟基化胆汁酸的酶法进行了评估，用于分析经硫酸酯水解后的尿液中的胆汁酸。将酶法获得的结果与通过放射免疫分析法对患者尿液样本中7α-羟基化胆汁酸获得的结果进行比较。酶法的结果还与在Sephadex-DEAP-LH-20上对胆汁酸进行分组分离后对单个胆汁酸进行气液色谱分析的结果进行了比较。酶法与放射免疫分析法在尿液胆汁酸测定方面存在显著相关性。与气液色谱法相比，胆汁酸分组分离后对不同结合物的分析未显示非特异性反应。7α-羟基化尿胆汁酸的参考范围与早期发表的结果一致。
