Farr B, Hackett S F, Winther B, Hendley J O
Acta Otolaryngol Suppl. 1984;413:15-8. doi: 10.3109/00016488409128536.
A method is described for measuring polymorphonuclear leukocyte (PMN) concentrations in expelled nasal mucus. Nasal mucus specimens (0.05-0.15 ml) were diluted 1:60 in Hank's balanced salt solution. Diluted mucus was vigorously agitated to disperse contained cells, and cells were counted in a hemocytometer. The mean PMN counts in nasal mucus from healthy young adults was 7.1 X 10(6) PMNs/ml mucus, but large daily fluctuations were observed in PMN counts in the same individual. This method may be useful to investigate inflammatory conditions of the nasal cavity.
本文描述了一种测量排出的鼻腔黏液中多形核白细胞（PMN）浓度的方法。将鼻腔黏液标本（0.05 - 0.15毫升）在汉克平衡盐溶液中按1:60稀释。对稀释后的黏液进行剧烈搅拌以分散其中所含的细胞，然后在血细胞计数器中对细胞进行计数。健康年轻成年人鼻腔黏液中的PMN平均计数为每毫升黏液7.1×10⁶个PMN，但在同一个体中观察到PMN计数存在较大的每日波动。该方法可能有助于研究鼻腔的炎症状况。
