血清中熊去氧胆酸的放射免疫测定

Radioimmunoassay of ursodeoxycholic acid in serum.

作者信息

Makino I, Tashiro A, Hashimoto H, Nakagawa S, Yoshizawa I

出版信息

J Lipid Res. 1978 May;19(4):443-7.

Abstract

A sensitivie and specific radioimmunoassay for the measurement of serum ursodeoxycholic acid has been developed. Ursodeoxycholic acid bound to bovine serum albumin was used as an antigen, and antiserum to this antigen was raised in the rabbit. [11, 12-3h2]Ursodeoxycholic acid was used as the radioactive tracer, and the radioimmunoassay was carried out by the method of Simmonds et al. (1973. Gastroenterology. 65: 705-711). The percentage of bound radioactivity decreased linearly with a logarithmic increase in unlabeled ursodeoxycholic acid from 10 to 200 pmol. The antiserum showed extremely high specificity for ursodeoxycholic acid (free and conjugated), and the values determined by radioimmunoassay indicated a close correlation with those found by gas-liquid chromatography. In normal Japanese subjects, a small amount of ursodeoxycholic acid in serum was detected, and the level was detected, and the level was 0.15 +/- 0.11 nmol/ml. This convenient radioimmunoassay will provide useful information about the metabolism of ursodeoxycholic acid in man.

摘要

已开发出一种用于测量血清熊去氧胆酸的灵敏且特异的放射免疫测定法。与牛血清白蛋白结合的熊去氧胆酸用作抗原，并在兔体内产生针对该抗原的抗血清。[11, 12 - 3h2]熊去氧胆酸用作放射性示踪剂，放射免疫测定法采用西蒙兹等人（1973年，《胃肠病学》，65: 705 - 711）的方法进行。随着未标记熊去氧胆酸从10皮摩尔对数增加至200皮摩尔，结合放射性百分比呈线性下降。该抗血清对熊去氧胆酸（游离型和结合型）显示出极高的特异性，放射免疫测定法测定的值与气液色谱法测定的值密切相关。在正常日本受试者中，检测到血清中有少量熊去氧胆酸，水平为0.15±0.11纳摩尔/毫升。这种便捷的放射免疫测定法将为人体熊去氧胆酸代谢提供有用信息。