一些羟基自由基诱导的溶菌酶产物的鉴定。

Identification of some OH radical-induced products of lysozyme.

作者信息

Dizdaroglu M, Gajewski E, Simic M G, Krutzsch H C

出版信息

Int J Radiat Biol Relat Stud Phys Chem Med. 1983 Feb;43(2):185-93. doi: 10.1080/09553008314550211.

Abstract

OH radical reactions with lysozyme in gamma-irradiated N2O saturated aqueous solutions caused formation of allo-threonine, alpha-amino-n-butyric acid, o- and m- tyrosines, and 2- and 3-hydroxytyrosines. These identified radiolytic products were characterized by capillary gas chromatography-mass spectrometry as their trimethylsilyl derivatives after HCl-hydrolysis of irradiated lysozyme. Their initial G-values were also determined using gas chromatography. The possible use of these radiolytic products as monitors of radiation-induced damage to proteins and the sites of attack are also discussed.

摘要

在经γ射线辐照的N₂O饱和水溶液中，羟基自由基与溶菌酶发生反应，生成了别苏氨酸、α-氨基正丁酸、邻酪氨酸和间酪氨酸，以及2-羟基酪氨酸和3-羟基酪氨酸。这些已鉴定出的辐射分解产物，在对辐照后的溶菌酶进行盐酸水解后，通过毛细管气相色谱-质谱联用仪，以其三甲基硅烷基衍生物的形式进行表征。还使用气相色谱法测定了它们的初始G值。文中还讨论了这些辐射分解产物作为蛋白质辐射损伤监测指标的可能性以及攻击位点。