Povey P M, Smith B R, Hall R
J Endocrinol. 1978 Jun;77(3):353-60. doi: 10.1677/joe.0.0770353.
The surface membrane proteins of cultured porcine thyroid cells have been labelled with 125I by the lactoperoxidase method. Evidence that the labelling was restricted to the cell surface was supported by the high viability of the cells in suspension, the high proportion of labelled material in the particulate fraction after homogenization and electron-microscopic autoradiographic studies. The labelled proteins were analysed by electrophoresis on polyacrylamide gels containing sodium dodecyl sulphate and this indicated the presence of ten major labelled protein bands with approximate molecular weights of 175 000, 155 000, 135 000, 88 000, 80 000, 52 300, 39 000, 30 000, 21 000 and 14 300. Comparison of the electrophoretic patterns obtained with cultured human and porcine thyroid cells suggested that there were species differences in the proportions of lower-molecular-weight proteins.
已通过乳过氧化物酶法用¹²⁵I标记培养的猪甲状腺细胞的表面膜蛋白。细胞在悬浮液中的高活力、匀浆后颗粒部分中标记物质的高比例以及电子显微镜放射自显影研究均支持标记仅限于细胞表面的证据。通过在含有十二烷基硫酸钠的聚丙烯酰胺凝胶上进行电泳分析标记的蛋白质，结果表明存在十条主要的标记蛋白带，其近似分子量分别为175000、155000、135000、88000、80000、52300、39000、30000、21000和14300。对培养的人甲状腺细胞和猪甲状腺细胞获得的电泳图谱进行比较表明，低分子量蛋白质的比例存在种属差异。
