Brennan A, Povey P M, Smith B R, Hall R
J Endocrinol. 1980 May;85(2):245-51. doi: 10.1677/joe.0.0850245.
Isolated porcine thyroid cells were surface-labelled with 125I using the lactoperoxidase technique. Samples of the cells were then cultured and harvested at various intervals for up to 7 days. The labelled proteins remaining on the cells or shed into the culture medium were analysed by electrophoresis on polyacrylamide gels run in sodium dodecyl sulphate. These studies indicated that the several different surface proteins of the thyroid cells were lost from the cell surface at similar rates (half-time of approximately 28 h) as the result, at least in part, of a process which depended on active cell metabolism. In addition, the gel profiles obtained from analysis of both medium and membrane-bound labelled proteins were similar and this suggested that peptide cleavage was not involved in the shedding of the majority of these proteins.
使用乳过氧化物酶技术，用¹²⁵I对分离出的猪甲状腺细胞进行表面标记。然后对细胞样本进行培养，并在长达7天的不同时间间隔进行收获。通过在十二烷基硫酸钠中运行的聚丙烯酰胺凝胶电泳分析留在细胞上或释放到培养基中的标记蛋白。这些研究表明，甲状腺细胞的几种不同表面蛋白以相似的速率（半衰期约为28小时）从细胞表面丢失，至少部分是由于一个依赖于活跃细胞代谢的过程。此外，从培养基和膜结合标记蛋白分析获得的凝胶图谱相似，这表明肽裂解不参与这些蛋白的大部分脱落过程。
