Feldman S P, Mertelsmann R, Venuta S, Andreeff M, Welte K, Moore M A
Blood. 1983 Apr;61(4):815-8.
The production of large quantities of Interleukin-2 (IL-2) from normal human lymphocytes has been limited by the short production and release period, as well as its absorption by the responsive cell population. We report the utilization of sodium azide (NaN3, 0.01% final concentration) to allow continued production of IL-2 as long as 72-96 hr and thereby increase the yield significantly. Cell cycle analysis performed by flow cytometry indicates that NaN3 blocks cells at the G0-G1 transition and the G1-S transition, depending on the time of addition of NaN3 to the cultures.
正常人类淋巴细胞产生大量白细胞介素-2（IL-2）受到生产和释放周期短以及反应性细胞群体对其吸收的限制。我们报告了利用叠氮化钠（NaN3，终浓度0.01%）使IL-2持续产生长达72 - 96小时，从而显著提高产量。通过流式细胞术进行的细胞周期分析表明，NaN3根据向培养物中添加NaN3的时间，将细胞阻滞在G0 - G1期转换和G1 - S期转换阶段。
