Schiller J, Arnhold J, Gründer W, Wagner M, Werner A, Arnold K
Institut für Medizinische Physik und Biophysik, Medizinische Fakultät, Universität Leipzig.
Biomed Tech (Berl). 1995 Sep;40(9):250-4. doi: 10.1515/bmte.1995.40.9.250.
The pseudohalogenide sodium azide (NaN3) and the organometallic mercury compound merthiolate (sodium salt of ethyl mercurithiosalicylate) are commonly used as preserving solutions for biomaterials. In the present paper, these preserving solutions were compared for their efficacy in preserving porcine articular cartilage. For this purpose, porcine articular cartilage was incubated with phosphate buffer containing the corresponding amounts of NaN3 or merthiolate. Supernatants were assayed using NMR spectroscopy, and the content of soluble saccharides was determined using the dye Alcian blue. The results obtained clearly indicate that merthiolate is more effective than sodium azide. This makes the former a more suitable preserving agent for the storage of cartilage. A significant decrease in the lactate concentration, and enhancement of the acetate and the N-acetyl resonances indicate degenerative changes in the cartilage. However, when a large excess of sodium azide is used, its efficacy approaches that of merthiolate.
拟卤化物叠氮化钠（NaN₃）和有机金属汞化合物硫柳汞（乙基汞硫代水杨酸钠盐）通常用作生物材料的保存溶液。在本文中，对这些保存溶液在保存猪关节软骨方面的功效进行了比较。为此，将猪关节软骨与含有相应量NaN₃或硫柳汞的磷酸盐缓冲液一起孵育。使用核磁共振光谱法分析上清液，并使用阿尔辛蓝染料测定可溶性糖的含量。所得结果清楚地表明硫柳汞比叠氮化钠更有效。这使得前者成为储存软骨更合适的保存剂。乳酸浓度的显著降低以及乙酸盐和N - 乙酰共振的增强表明软骨发生了退行性变化。然而，当使用大量过量的叠氮化钠时，其功效接近硫柳汞。
