Schuessler H, Davies J V
Int J Radiat Biol Relat Stud Phys Chem Med. 1983 Mar;43(3):291-301. doi: 10.1080/09553008314550331.
The reduction of bovine serum albumin (BSA) by hydrated electrons, formate and ethanol radicals leads to the formation of S-S-radicals. The reaction of the hydrated electrons becomes faster, if BSA was preirradiated in the presence of formate or ethanol, but the rate does not change if radiolysis is done in the presence of t-butanol or without scavenger. The reduction of BSA by formate radicals is 10 times slower but gives a higher yield of S-S-radicals than by hydrated electrons. The rate and yield of the reduction by the formate radicals are increased by preirradiation of BSA. The reduction by ethanol radicals is five times slower than by formate radicals and gives lower yields of S-S-radicals which are increased by preirradiation. All rates and yields of the reduction reactions are decreased by changing the pH from 6.3 to 7.3.
水合电子、甲酸根和乙醇自由基对牛血清白蛋白（BSA）的还原作用会导致S-S自由基的形成。如果BSA在甲酸根或乙醇存在下进行预辐照，水合电子的反应会加快，但在叔丁醇存在下或无清除剂时进行辐解，反应速率不变。甲酸根自由基对BSA的还原作用比水合电子慢10倍，但产生的S-S自由基产率更高。预辐照BSA可提高甲酸根自由基还原的速率和产率。乙醇自由基的还原作用比甲酸根自由基慢5倍，产生的S-S自由基产率较低，预辐照可提高该产率。将pH从6.3变为7.3会降低所有还原反应的速率和产率。
