Mölders H H, Breuning M H, Ivanyi P, Ploegh H L
Hum Immunol. 1983 Feb;6(2):111-7. doi: 10.1016/0198-8859(83)90067-8.
Biochemical analysis of HLA-B27 antigens from individuals that are HLA-ABC identical by serology but distinct in CML typing establishes that two types of HLA-B27 can be defined biochemically. The division of HLA-B27 into a W type and a K type by CML typing correlates perfectly with the biochemical data. HLA-B27 K type possesses a more basic isoelectric point than HLA-B27 W type. Neuraminidase digestions of immunoprecipitated HLA-A,B antigens establish that the difference in isoelectric point between HLA-B27 K and HLA-B27 W is not due to differences in sialic acid content.
对血清学上HLA - ABC相同但CML分型不同的个体的HLA - B27抗原进行生化分析，结果表明可通过生化方法定义两种类型的HLA - B27。通过CML分型将HLA - B27分为W型和K型，这与生化数据完全相关。HLA - B27 K型的等电点比HLA - B27 W型更偏碱性。对免疫沉淀的HLA - A、B抗原进行神经氨酸酶消化，结果表明HLA - B27 K型和HLA - B27 W型之间等电点的差异并非由于唾液酸含量的不同。
