Grumet F C, Pask S B, Ness D B, Fendly B M, Engleman E G
Hum Immunol. 1983 Feb;6(2):63-73. doi: 10.1016/0198-8859(83)90062-9.
A new modification of an HLA-DR typing technique is described which makes DR typing as rapid and simple as routine HLA-A,B,C typing. In this new method, designated the TM1 technique, carboxyfluoresceindiacetate labeled peripheral blood lymphocytes are added directly to DR typing trays. The T cells are then lysed by addition of TM1, a pan-T cytotoxic IgM monoclonal antibody, and residual B-cell reactivity with cytotoxic DR alloantibodies is read as in routine fluorochromasia microlymphocytotoxicity. HLA-DR typing by the TM1 technique compares favorably to typing by methods using B cells enriched by sheep red blood cell rosetting or by Degalan bead columns. The TM1 technique also works well with cells that have been cryopreserved as well as with cells that have been separated from whole blood drawn as much as 3 days earlier. Finally, because TM1 is so effective in lysing normal T lymphocytes, this antibody may prove useful in functional in vitro and in vivo studies requiring T-cell depletion.
本文描述了一种HLA - DR分型技术的新改良方法，该方法能使DR分型像常规HLA - A、B、C分型一样快速简便。在这种名为TM1技术的新方法中，将羧基荧光素二乙酸酯标记的外周血淋巴细胞直接加入到DR分型板中。然后通过加入TM1（一种泛T细胞毒性IgM单克隆抗体）裂解T细胞，剩余B细胞与细胞毒性DR同种异体抗体的反应性则按照常规荧光显微镜微量淋巴细胞毒性试验进行读取。通过TM1技术进行的HLA - DR分型与使用经绵羊红细胞花环富集的B细胞或Degalan珠柱法进行分型的效果相当。TM1技术对于冷冻保存的细胞以及早在3天前采集的全血中分离出的细胞同样适用。最后，由于TM1在裂解正常T淋巴细胞方面非常有效，这种抗体可能在需要去除T细胞的体外和体内功能研究中证明是有用的。
