Lee J H, Lias M, Deng C T, Etessami S, Loon J, Chen M, Banh L, Conger N, Connors D, Manzo A
One Lambda, Inc., Canoga Park, CA.
Tissue Antigens. 1994 Jul;44(1):34-42. doi: 10.1111/j.1399-0039.1994.tb02354.x.
We have developed monoclonal antibodies to most HLA specificities, making it possible for us to devise a simple, rapid, one-step microcytotoxicity test. The test is performed by adding 1 microliter of cells to 1 microliter of antibody-complement mixture predotted on the microtest tray. The reactions are read following a 1-hour incubation period (30 minutes in some instances). The analysis of reactions seen on testing 105 class I antibodies and 50 class II antibodies is shown. A comparison of typing by the standard NIH method and the new one-step procedure showed a > 96% concordance in the 500 T cells and 200 B cells we examined. Class I and class II typing could be performed using B cells, thus obviating the need to isolate both T and B cells for HLA typing.
我们已经研制出针对大多数HLA特异性的单克隆抗体，这使我们能够设计出一种简单、快速的一步法微量细胞毒性试验。该试验通过将1微升细胞加入到预先点在微量试验板上的1微升抗体 - 补体混合物中来进行。在孵育1小时后（某些情况下为30分钟）读取反应结果。文中展示了对105种I类抗体和50种II类抗体检测反应的分析。在我们检测的500个T细胞和200个B细胞中，通过标准国立卫生研究院方法和新的一步法进行分型的比较显示一致性超过96%。使用B细胞即可进行I类和II类分型，因此无需为进行HLA分型而同时分离T细胞和B细胞。
