Nakanishi K, Howard M, Muraguchi A, Farrar J, Takatsu K, Hamaoka T, Paul W E
J Immunol. 1983 May;130(5):2219-24.
Highly purified B lymphocytes cultured with affinity-purified goat anti-mouse IgM antibodies and B cell growth factor (BCGF) proliferate but fail to differentiate into immunoglobulin-synthesizing cells during 4-day cultures. Induction of immunoglobulin (Ig) synthesis depends on the presence of two additional T cell-derived factors (T cell-replacing factors [TRF]). One TRF, designated B15 1K 12-TRF, is found in the supernatant of B15 1K 12 T hybridoma cells. It is required relatively early in these cultures and appears to cause an increase in cell yield as well as Ig synthesis. The second factor, designated EL-TRF, is found in PMA-induced EL-4 supernatant. It appears to be different from both BCGF and IL 2 in that it can be separated from the former by isoelectric focusing and from the latter by phenyl-Sepharose chromatography. EL-TRF is still active if added on the last day of a 4-day culture. A model for the action of anti-IgM and these three T cell-derived factors in B cell activation and differentiation is proposed.
用亲和纯化的山羊抗小鼠IgM抗体和B细胞生长因子（BCGF）培养的高度纯化B淋巴细胞在4天培养期间会增殖，但无法分化为免疫球蛋白合成细胞。免疫球蛋白（Ig）合成的诱导取决于另外两种T细胞衍生因子（T细胞替代因子[TRF]）的存在。一种TRF，称为B15 1K 12-TRF，存在于B15 1K 12 T杂交瘤细胞的上清液中。在这些培养中它在相对早期就需要，并且似乎会导致细胞产量以及Ig合成增加。第二种因子，称为EL-TRF，存在于PMA诱导的EL-4上清液中。它似乎与BCGF和IL-2都不同，因为它可以通过等电聚焦与前者分离，通过苯基琼脂糖凝胶层析与后者分离。如果在4天培养的最后一天添加，EL-TRF仍然有活性。本文提出了抗IgM和这三种T细胞衍生因子在B细胞活化和分化中的作用模型。
