Koyama N, Harada N, Takahashi T, Mita S, Okamura H, Tominaga A, Takatsu K
Department of Biology, Kumamoto University Medical School, Japan.
Immunology. 1988 Feb;63(2):277-83.
The B-cell differentiation-inducing activity of interleukin-1 (IL-1) was compared with that of T-cell replacing factor (TRF)/interleukin-5 (IL-5), which was originally described as a late-acting B-cell differentiation-inducing factor. Human recombinant IL-1 and murine recombinant TRF/IL-5 were used in this study. Purified B cells from non-primed or antigen-primed mice, LPS-stimulated B-cell blasts, and chronic B-cell leukaemia (BCL1) cells were used as the responding B-cell population. Addition of IL-1 to the culture of normal B-cells and sheep red blood cells (SRBC) induced a dose-dependent anti-SRBC IgM response, with maximal response at 100 U/ml, whereas the response induced by TRF/IL-5 was less than that induced by IL-1 and did not reach the maximum even at 100 U/ml. Addition of anti-IL-1 antibody, but not anti-TRF/IL-5 antibody or anti-IL-2 receptor antibody, inhibited IL-1-induced anti-SRBC responses. Depletion of cells adherent to Sephadex beads from splenic B cells showed no significant effect on the magnitude of the total responses. IL-1 could induce little, if any, differentiation in antigen-primed B cells, LPS-stimulated B-cell blasts, or BCL1 cells into antibody-secreting cells, whereas differentiation could be induced by low doses of TRF/IL-5 (1-2 U/ml). Of great interest is that suboptimal doses of IL-1 (10 U/ml) could synergize with TRF in the primary anti-SRBC PFC responses. Kinetic studies revealed that IL-1 acts on B cells for the first 2 days and TRF/IL-5 for the last 3 days in 5-day cultures of B cells. These results suggest that IL-1 acts primarily on resting B cells as a differentiation-inducing factor in the presence of antigen, and also acts as a 'priming' factor for TRF/IL-5.
将白细胞介素-1（IL-1）的B细胞分化诱导活性与T细胞替代因子（TRF）/白细胞介素-5（IL-5）的活性进行了比较，TRF/IL-5最初被描述为一种晚期作用的B细胞分化诱导因子。本研究使用了人重组IL-1和鼠重组TRF/IL-5。来自未致敏或抗原致敏小鼠的纯化B细胞、脂多糖刺激的B细胞母细胞以及慢性B细胞白血病（BCL1）细胞用作反应性B细胞群体。向正常B细胞和绵羊红细胞（SRBC）的培养物中添加IL-1可诱导剂量依赖性的抗SRBC IgM反应，在100 U/ml时反应最大，而TRF/IL-5诱导的反应小于IL-1诱导的反应，即使在100 U/ml时也未达到最大值。添加抗IL-1抗体可抑制IL-1诱导的抗SRBC反应，而抗TRF/IL-5抗体或抗IL-2受体抗体则无此作用。从脾B细胞中去除黏附于葡聚糖凝胶珠的细胞对总反应的幅度无显著影响。IL-1几乎不能诱导抗原致敏的B细胞、脂多糖刺激的B细胞母细胞或BCL1细胞分化为抗体分泌细胞，而低剂量的TRF/IL-5（1-2 U/ml）可诱导分化。非常有趣的是，次优剂量的IL-1（10 U/ml）可在初次抗SRBC PFC反应中与TRF协同作用。动力学研究表明，在B细胞5天的培养中，IL-1在前2天作用于B细胞，TRF/IL-5在后3天作用于B细胞。这些结果表明，IL-1在有抗原存在的情况下主要作为分化诱导因子作用于静止B细胞，并且还作为TRF/IL-5的“启动”因子。
